Cancelled Advanced Methods for the Analysis of Protein Disease Mechanisms - elective
Course content
Through introductory lectures, student lead seminars, demonstrations and lab exercises this course aims at preparing students for a career in biomedical research. It brings together a group of experienced researchers and teachers to provide an overview of several advanced methodologies used to characterize protein mechanisms of action in living cells.
MSc Programme in Human Biology - Elective
After completing the course the student is expected to:
Knowledge
- Explain the rationale for the utilization of several advanced
research methodologies to characterize protein mechanisms of action
“in vivo” with a focus on 4 general areas:
- 1) mass spectrometry based methodologies for quantitative analysis of proteomes and its posttranslational modifications,
- 2) light microscopy based analysis of protein signaling and protein-protein interactions,
- 3) gene editing and
- 4) the use of chromatin immuneprecipitation and immunocytochemistry-FISH for the analysis protein regulation of DNA/RNA metabolism.
- Explain the principles of antibody based methods to study chromatin regulation and function in living cells and tissues.
- Understand the principles of mass spectrometry based methodologies applied to protein analysis and its applications to cell signaling studies, including isotope labeling for MS-based quantitation.
- Explain sample preparation methods for the analysis of protein posttranslational modifications.
- Understand the basis for the interpretation of MS/MS spectra
- Explain the physical principles that govern the use of light microscopy methods for the characterization of proteins in living cells.
- Identify the different microscopy methods commonly used for analysis of cellular protein signaling events, including the use of advanced imaging analysis and live cell imaging.
- Understand the basis for the analysis of protein-protein interactions in living cells using advanced microscopy techniques such as FRAP and FRET.
- Identify advantages and limitations of the use of above listed methods.
Skills
- Interpret the experimental data obtained by the above mentioned methods.
- Present experimental data in an understandable and scientifically sound manner.
- Discuss and critically review articles and research proposal for appropriateness in the utilization of methodological approaches for characterization of protein function in vivo.
- Design a tailored research plan to test specific hypotheses related to the characterization of protein function in mammalian cells using relevant and informative methods.
Competencies
The students will develop competencies in:
- project planning,
- data acquisition and interpretation
- scientific communication both written and oral.
The course is comprised of two modules: an e-learning module comprising online lectures, theoretical exercises and literature reviews and a classroom learning module lasting two weeks mainly comprising student-led seminars, lab exercises and demonstrations.
A completed Bachelor degree within the Medical, Pharmaceutical or Natural Sciences. The students must be able to read and write English at a relevant scientific level.
- ECTS
- 5 ECTS
- Type of assessment
-
Requirement to attend classesWritten assignment, 72 hours
- Type of assessment details
- Completion of e-learning modules and approved participation in
the teaching and training activities
The written assignment will be in the form of a 2 pages long essay. The student will have 72 hours to complete the assignment and submit the essay. - Exam registration requirements
-
None
- Aid
- All aids allowed
- Marking scale
- passed/not passed
- Censorship form
- No external censorship
Internal examiners
Criteria for exam assessment
To achieve the assesment Passed, the student must adequately be able to:
Knowledge
- Complete most of their practical and theoretical exercises in a timely and satisfactory manner.
- Demonstrate an understanding of the methods used for gene editing in mammalian cell and animal models
- Demonstrate an understanding of advanced methodologies to study protein-DNA interactions in living cells or tissues
- Demonstrate an understanding of the principles of mass spectrometry applied to protein analysis.
- Demonstrate an understanding of the utilization of high-end quantitative proteomics technologies in the characterization of biological systems, with a focus on stable isotope labeling and high-resolution mass spectrometry.
- Demonstrate understanding of light microscopy methods for the functional characterization of proteins in living cells.
- Analyze and evaluate the utilization of microscopy for the characterization of protein-protein interactions in living cells.
Skills
- Critical assesment of research reports and proposals for the correct utilization of the methods covred in the course, including data interpretation.
- Design a coherent research plan to test specific hypotheses related to the characterization of protein function in mammalian cells using relevant and informative methods
Single subject courses (day)
- Category
- Hours
- Lectures
- 18
- Preparation
- 32
- Practical exercises
- 48
- Seminar
- 15
- Exam
- 24
- English
- 137
Kursusinformation
- Language
- English
- Course number
- SHUA13021U
- ECTS
- 5 ECTS
- Programme level
- Full Degree Master
- Duration
-
1 block
- Placement
- Block 2
- Price
-
- Schedulegroup
-
BE-learning module:
Classroom teaching:
Examination (72 hours): - Capacity
- 20 particpants
- Studyboard
- Study Board for Human Biology, Immunology and Neuroscience
Contracting department
- Center for Protein Research
Contracting faculty
- Faculty of Health and Medical Sciences
Course Coordinator
- Jesper Velgaard Olsen (12-6d687673687531726f76687143667375316e7831676e)
Teacher
Prof. Jesper V. Olsen, Prof. Michael L. Nielsen, Prof. Chunaram Choudhary, Prof. Nils Mailand, Prof. Claudia Lukas, Prof. Guillermo Montoya, Ass.Prof. Jeremy A. Daniel, Ass.Prof. Jakob Nilsson
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