Cancelled Principles and Practice of Bioanalysis
Course content
The course is intended for (a) students specializing in bioanalysis (determination of pharmaceuticals in biological fluids), for (b) students with need for improved general knowledge and skills in analytical chemistry / pharmaceutical chemical analysis, and for (c) students specializing in drug metabolism. Students according to (b) and (c) are typically master students using analytical techniques during the experimental work for the master thesis. The course focuses both on theoretical and practical aspects of bioanalysis.
The first part of the course teaches the principles of major bioanalytical techniques at high level (lectures):
- Sample preparation (protein precipitation, liquid extraction, solid-phase extraction)
- Liquid chromatography
- Liquid chromatography-mass spectrometry
- Gas chromatography
- Gas chromatography-mass spectrometry
- Capillary electrophoresis
The second part of the course teaches the use of the above techniques at high level for the following purposes (lectures):
- Determination of small molecule pharmaceuticals and their metabolites in biological fluids (blood, urine, saliva, dried blood spots)
- Determination of biopharmaceuticals in biological fluids
- In-vitro metabolism studies of small molecule pharmaceuticals
Application areas include therapeutic drug monitoring, in-vitro metabolism studies, forensic toxicology, and doping analysis.
The third part of the course focus on reading and understanding research articles in bioanalysis related to the techniques and applications outlined above, and include student presentation of selected articles.
The fourth part of the course comprises laboratory exercises related to the techniques and applications outlined above.
MSc Programme in Pharmacy (Danish programme cand.pharm) - elective
MSc Programme in Pharmaceutical Sciences (Danish programme cand.scient.pharm) - restricted elective
MSc Programme in Medicinal Chemistry - elective
MSc Programme in Pharmaceutical Sciences (English programme)- restricted elective
MSc Programme in Environmental Science (SCIENCE) - restricted elective
At the end of the course, students are expected to be able to:
Knowledge
- Have an in-depth theoretical understanding of the major techniques used in bioanalysis of pharmaceuticals and their metabolites
- Understand the individual steps in procedures for determination of small molecule pharmaceuticals and biopharmaceuticals in biological fluids
- Understand the individual steps of in-vitro metabolism studies of small molecule pharmaceuticals
Skills
- Be able to perform practical bioanalytical work according to published / existing procedures
- Know and be able to use a broad number of separation and detection principles as well as sample preparation techniques used for bioanalysis.
- Understand the principles behind and use the analytical chemical instrumentation needed for high sensitivity analysis of samples of biological origin.
- Judge and use the relevant original analytical chemical literature, handbooks and databases.
Competences
- Understand and perform bioanalytical chemical work.
- Develop new analytical chemical methods for analysis of samples of biological origin (e.g. urine, plasma, serum, faeces, saliva, synovial liquid, plant materials etc.).
- Design sampling and storage protocols for in vivo studies of drug metabolism.
Lectures, seminars, students presentations and, laboratory exercises
- Steen Honore Hansen, Stig Pedersen-Bjergaard: Bioanalysis of Pharmaceuticals: Sample Preparation, Chromatography and Mass Spectrometry, ISBN: 978-1-118-71682-3, Wiley (2015)
- Additional course materials such as manuals and scientific papers are availabe from the course homepage
Course teaching is based upon the assumption that the student has general knowledge within the areas of organic chemistry, inorganic chemistry, physical chemistry, analytical chemistry, biochemistry, pharmacology, toxicology, and drug development at bachelor level.
If you are applying for the course as a credit transfer student, you must have passed SFAB20035U Farmaceutisk analytisk kemi or have acquired similar competencies in another course. Documentation for corresponding competencies in the form of a course description and an exam result must be attached to your application.
Open for credit transfer students and other external students. Apply here:
Credit transfer students:
Credit transfer student at SUND – University of Copenhagen
(ku.dk)
Other external students:
https://healthsciences.ku.dk/education/student-mobility/guest-students/
Credit transfer and other external students are welcomed on the course if there are seats available and they have the academic qualifications.
- ECTS
- 2,5 ECTS
- Type of assessment
-
Continuous assessment
- Type of assessment details
- In order to obtain the course certificate the student should participate actively during laboratory exercises, complete all the laboratory exercises and make an oral satisfying presentation of the "Independent Study Activity".
- Marking scale
- passed/not passed
- Censorship form
- No external censorship
Criteria for exam assessment
To achieve a course certificate the student must be able to:
Knowledge
- Have an in-depth theoretical understanding of the major techniques used in bioanalysis of pharmaceuticals
- Understand the individual steps in procedures for determination of small molecule pharmaceuticals and biopharmaceuticals in biological fluids
- Understand the individual steps of in-vitro metabolism studies of small molecule pharmaceuticals
Skills
- Be able to perform practical bioanalytical work according to published / existing procedures
- Know and be able to use a broad number of separation and detection principles as well as sample preparation techniques used for bioanalysis.
- Understand the principles behind and use the analytical chemical instrumentation needed for high sensitivity analysis of samples of biological origin.
- Judge and use the relevant original analytical chemical literature, handbooks and databases.
Competences
- Understand and perform bioanalytical chemical work.
- Develop new analytical chemical methods for analysis of samples of biological origin (e.g. urine, plasma, serum, faeces, saliva, synovial liquid, plant materials etc.).
- Design sampling and storage protocols for in vivo studies of drug substances.
In the "Exam 1" the students are evaluated from the participation in the laboratory exercises as well their "Independent Study Activity" where they critically present analytical chemical literature.
- ECTS
- 5 ECTS
- Type of assessment
-
On-site written exam, 2 hours under invigilation
- Type of assessment details
- Examination type:
2 hour multiple-choice test. The students are evaluated in a multiple choice examination that covers questions related to the lab exercices, lectures and student presentations. - Aid
- Written aids allowed
Find more information about written on-site exams in the exam rooms, incl. information about standard programs on the exam PCs at KUnet
In addition to the standard programs digital notes are permitted for this exam. It is allowed to upload notes for the ITX exam via digital exam. You will find a link to this feature from your exam in Digital Exam.
- Marking scale
- passed/not passed
- Censorship form
- No external censorship
- Re-exam
-
10 or fewer students registered for reexam:
Type of assessment: Oral examination
Assessment details: 20 minutes examination
Preparation: 30 minutes
Aids: Written aids (i.e. printed notes, books, etc. that you have with you)
Criteria for exam assessment
To pass the course the student must be able to:
Knowledge
- Have an in-depth theoretical understanding of the major techniques used in bioanalysis of pharmaceuticals
- Understand the individual steps in procedures for determination of small molecule pharmaceuticals and biopharmaceuticals in biological fluids
- Understand the individual steps of in-vitro metabolism studies of small molecule pharmaceuticals
Skills
- Be able to perform practical bioanalytical work according to published / existing procedures
- Know and be able to use a broad number of separation and detection principles as well as sample preparation techniques used for bioanalysis.
- Understand the principles behind and use the analytical chemical instrumentation needed for high sensitivity analysis of samples of biological origin.
- Judge and use the relevant original analytical chemical literature, handbooks and databases.
Competences
- Understand and perform bioanalytical chemical work.
- Develop new analytical chemical methods for analysis of samples of biological origin (e.g. urine, plasma, serum, faeces, saliva, synovial liquid, plant materials etc.).
- Design sampling and storage protocols for in vivo studies of drug substances.
Single subject courses (day)
- Category
- Hours
- Lectures
- 37
- Preparation
- 132
- Practical exercises
- 24
- Seminar
- 13
- English
- 206
Kursusinformation
- Language
- English
- Course number
- SFAK20014U
- ECTS
- See exam description
- Programme level
- Full Degree Master
Full Degree Master choice
- Duration
-
1 block
- Placement
- Block 1
- Schedulegroup
-
C
- Capacity
- 40 students
- Studyboard
- Study Board of Pharmaceutical Sciences
Contracting department
- Department of Pharmacy
Contracting faculty
- Faculty of Health and Medical Sciences
Course Coordinators
- Nickolaj J. Petersen
(17-716c666e726f646d3173687768757668714376787167316e7831676e)
Main responsible - Stig Pedersen-Bjergaard (19-75307267666774756770646c677469636374664275777066306d7730666d)
Teacher
Stig Pedersen-Bjergaard
Nickolaj J. Petersen
Kasper Dyrberg Rand
Jörg P Kutter
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