Advanced Fluorescence Spectroscopy and Microscopy

Course content

The course focus on the theoretical background for and applications of fluorescence spectroscopy and microscopy. The course deals with photophysical properties of molecular systems, in particular related to UV-Vis absorption and fluorescence. Special emphasis is on: energy and electron transfer, polarization (anisotropy), and solvent effects, single molecule spectroscopy and super resolution imaging, time resolved fluorescence and transient absorption. 


MSc Programme in Chemistry

MSc Programme in Chemistry with a minor subject

MSc Programme in Nanoscience

Learning outcome

After having passed the course, the student should be able to:



Concepts of

  • the interaction of light with molecules
  • photo physical manifestations of the local molecular environment
  • for the kinetics involved in deactivation of electronically excited states of molecules
  • the basics of photo induced electron transfer
  • steady-state and time-resolved anisotropy of fluorescence emission
  • the principles of steady-state and time-resolved experimental techniques
  • resonance energy transfer and its applications
  • application of fluorescence techniques in sensors
  • transient absorption
  • single molecule fluorescence techniques
  • super resolution fluorescence imaging



  • Apply the above mentioned knowledge for understanding and calculating photophysical properties of molecular systems.



  • Read contemporary research papers on photophysics, understand them, present them and write an self consistent essay on it.

Lectures, student presentations, discussions, and preparation individually and/or in groups.

  • Joseph R. Lakowicz: "Principles of Fluorescence Spectroscopy", 3rd edition.
  • Lecture notes.
  • Selected research articles.

Photophysic and physical chemistry at a level corresponding to the BSc courses KemiFoto (Photochemistry and Photophysics) or KemiBin (Chemical Bonding), KemiKS (Quantum Chemistry and Theoretical Spectroscopy)

Academic qualifications equivalent to a BSc degree is recommended.

7,5 ECTS
Type of assessment
Oral exam on basis of previous submission, 25 minutes (no preparation time)
Type of assessment details
Oral exam based on the written essay of the student, the material from the assignment, and the general curriculum of the course.
Submission of written essay before deadline: 5 page essay on specific topic and research papers (assignment). To be submitted 7 days after assignment has been posted on Digital Exam.
Without aids
Marking scale
7-point grading scale
Censorship form
No external censorship
Several internal examiners

Same as the ordinary exam.

A new 5 page essay on specific topic based on curriculum and research papers will be assigned, to be submitted 5 days after assignment.

Criteria for exam assessment

See learning outcomes

Single subject courses (day)

  • Category
  • Hours
  • Lectures
  • 18
  • Class Instruction
  • 18
  • Preparation
  • 90
  • Project work
  • 40
  • Exam
  • 40
  • English
  • 206


Course number
7,5 ECTS
Programme level
Full Degree Master

1 block

Block 4
No limitation – unless you register in the late-registration period (BSc and MSc) or as a credit or single subject student.
Study Board of Physics, Chemistry and Nanoscience
Contracting department
  • Department of Chemistry
Contracting faculty
  • Faculty of Science
Course Coordinator
  • Bo Wegge Laursen   (3-6a7f74486b706d7536737d366c73)

Tom Vosch
Bo Wegge Laursen

Saved on the 14-02-2024

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