Gene Technology (Gentek)
Course content
The theoretical part includes:
- A thorough review of gene technology methods
- Cellular control of macromolecular biosynthesis with emphasis on the experimental basis
- Generation of genome- and cDNA libraries
- Screening of libraries including expression cloning in Xenopus oocytes and micro organisms
- Genome-, trancriptome- and proteome analysis
- Production of transgenic animals
- Heterologous expression in bacteria, yeast/fungi, insect cells, mammalian cells and whole animals
The practical part includes:
- The most important methods to generate recombinant plasmids using restriction enzymes, Gateway® technology or homologous recombination
- Site-directed mutagenesis
- Real-time PCR for mRNA quantification and SNP analysis
- Application of reporter genes to study gene regulation and subcellular protein localisation
- Yeast 2-hybrid analysis
- Affinity purification of fusion proteins
- Pull-down experiments
- Fluorescence microscopy
- Internet based gene technology tools
- Danish and EU law on gene technology
BSc Programme in Biochemistry
BSc Programme in Bioinformatics
BSc Programme in Biology
Knowledge:
The course provides the student with a detailed and comprehensive knowledge on theoretical and practical modern gene technology. The student gets aquainted with practical laboratory routines fulfilling the requirements stated by the Danish and EU law on gene technology.
Skills:
The student gains core competences within theoretical and practical
modern gene technology. The degree of confidence with modern gene
technology/molecular biology obtained during the course allows
him/her to design gene technology/molecular biology experiments
based upon known gene technological methods and internet based
tools. The student obtains experience with critical interpretation
and treatment of original molecular biology data, and gets
acquainted with work routines in a gene technology laboratory in
relation to Danish and EU law on gene technology and genetically
modified organisms (GMOs).
Practical: The student gets aquainted with practical laboratory
routines fulfilling the requirements stated by the Danish and EU
law on gene technology.
Personal: The student learns to collaborate in groups and prepare a
written presentation and discussion of their obtained experimental
data.
Competencies:
The competencies obtained by the student include
- to interpret data from the scientific literature and put them in a molecular biology/gene technology context
- to design their own experiments
- to work in a classified laboratory in accordance with legal regulations
- to work in small teams
- to give a detailed description of and evaluate the gene technology tools presented during the course
- to apply the presented tools to design experiments with the potential to answer defined molecular biology questions
- to describe and explain individual steps in a presented gene technology manual
Lectures, problem solving and laboratory experiments.
See Absalon.
Knowledge corresponding to the content of the courses: Basic Biochemistry, Basic Molecular Biology and Basic Cell Biology.
The course is offered to students of Biology, Biochemistry, Molecular Biomedicine, Nanotechnology, Sports Science and related educations.
- ECTS
- 15 ECTS
- Type of assessment
-
On-site written exam, 4 hours under invigilation
- Type of assessment details
- The on-site written exam is an ITX exam.
See important information about ITX-exams at Study Information, menu point: Exams -> Exam types and rules -> Written on-site exams (ITX) - Aid
- All aids allowed
The University will make computers available to students at the ITX-exam.
Students are not permitted to bring digital aids like computers, tablets, calculators, mobile phones etc.
Books, notes, and similar materials can be brought in paper form or uploaded before the exam and accessed digitally from the ITX computer. Read more about this at Study Information.
- Marking scale
- 7-point grading scale
- Censorship form
- No external censorship
One internal examiner
- Re-exam
-
The same as the ordinary exam.
Criteria for exam assessment
In order to obtain the grade 12 the student should convincingly and accurately demonstrate the knowledge, skills and competences described under Learning Outcome.
Single subject courses (day)
- Category
- Hours
- Lectures
- 28
- Class Instruction
- 28
- Preparation
- 254
- Practical exercises
- 90
- E-Learning
- 8
- Exam
- 4
- English
- 412
Kursusinformation
- Language
- English
- Course number
- NBIB10009U
- ECTS
- 15 ECTS
- Programme level
- Bachelor
- Duration
-
1 block
- Placement
- Block 1
- Schedulegroup
-
All schedules
- Capacity
- No limitation – unless you register in the late-registration period (BSc and MSc) or as a credit or single subject student.
- Studyboard
- Study Board for the Biological Area
Contracting department
- Department of Biology
Contracting faculty
- Faculty of Science
Course Coordinator
- Per Amstrup Pedersen (10-7263726766677475677042646b71306d7730666d)
Teacher
Rasmus Hartmann-Petersen og Henriette Pilegaard
Se skema
Er du BA- eller KA-studerende?
Kursusinformation for indskrevne studerende