Translational Tools II – New Methods

Course content

The main themes covered in the course include:

  • Stem cells and induced pluripotent stem cells, and their applications in disease modeling
  • Precision nuclease-based genome editing
  • Proteolysis targeting chimera (PROTACs)
  • Mass spectrometry for understanding drug mechanisms
  • High resolution microscopy
  • Cryo-EM
Education

BRIDGE - Translational Excellence Programme

Learning outcome

Upon completing the course, participants should be able to:

 

Knowledge

Explain the rationale for the utilization of several advanced research methodologies to characterize protein mechanisms of action in vivo with a focus on three general areas:

  • Gene editing
  • PROTACs
  • Cryo-EM
  • Discuss the applications of mass spectrometry-based proteomic approaches for drug target discovery.

 

Skills

  • Culture and use mouse embryonic stem cells
  • Design of constructs for CRISPR-based gene targeting
  • Identifying targets of PROTACs using mass spectrometry
  • Basic principles and application of light microscopy and cryo-EM for structure/function analysis of proteins.

 

Competences

  • Identify and evaluate advantages and limitations of the use of above listed methods.
  • Justify the use of PROTACs and mass spectrometry in understanding the mechanisms of small molecule-based drugs or drug candidates
  • Understand the central aspects of translational tools and be able to discuss and communicate these to other scientists, clinicians, and the public.

The course will be organized with a mix of scientific seminars by invited speakers including lectures about stem cells, PROTACs, CRISPR-based genome engineering, high-resolution microscopy, cryo-EM, as well as the applications of modern proteomics technologies for translational research.

In addition, the course will include group work, demonstrations, and practical exercises supervised by leading specialists in the field.

The course will end with an evaluation where participants must reflect on course learning outcomes and give feedback for course development.

Course literature will be published on Absalon.

Participants must meet the admission criteria in BRIDGE - Translational Excellence Programme.

Oral
Continuous feedback during the course of the semester
ECTS
0 ECTS
Type of assessment
Continuous assessment
Course participation
Type of assessment details
Attendance and active participation
Aid
All aids allowed
Marking scale
passed/not passed
Censorship form
No external censorship
Criteria for exam assessment

Active contribution and course participation according to the BRIDGE Guidelines and Practicalities.

Part time Master and Diploma courses

  • Category
  • Hours
  • Lectures
  • 10
  • Preparation
  • 5
  • Theory exercises
  • 5
  • Exercises
  • 20
  • English
  • 40

Kursusinformation

Language
English
Course number
SBRI19011U
ECTS
0 ECTS
Programme level
Part Time Master
Ph.D.
Placement
Autumn
Schedulegroup
See course dates and course programme in Absalon.

*This course is offered in autumn 2024 for the BRIDGE 2023-2025 programme.
Capacity
15 participants
Studyboard
Study Board for the Professionel Master´s Degree Programmes at The Faculty og Health and Medical Science
Contracting department
  • Center for Protein Research
Contracting faculty
  • Faculty of Health and Medical Sciences
Course Coordinators
  • Chuna Ram Choudhary   (15-7176837c6f3c71767d8372766f80874e717e803c79833c7279)
  • Takeo Narita   (12-7f6c76707a39796c7d747f6c4b6e7b7d397680396f76)
  • Amelia Green   (12-6c787077746c39727d7070794b6e7b7d397680396f76)
Saved on the 05-07-2023

Are you BA- or KA-student?

Are you bachelor- or kandidat-student, then find the course in the course catalog for students:

Courseinformation of students