Translational Tools II – New Methods
Course content
The main themes covered in the course include:
- Stem cells and induced pluripotent stem cells, and their applications in disease modeling
- Precision nuclease-based genome editing
- Proteolysis targeting chimera (PROTACs)
- Mass spectrometry for understanding drug mechanisms
- High resolution microscopy
- Cryo-EM
BRIDGE - Translational Excellence Programme
Upon completing the course, participants should be able to:
Knowledge
Explain the rationale for the utilization of several advanced research methodologies to characterize protein mechanisms of action in vivo with a focus on three general areas:
- Gene editing
- PROTACs
- Cryo-EM
- Discuss the applications of mass spectrometry-based proteomic approaches for drug target discovery.
Skills
- Culture and use mouse embryonic stem cells
- Design of constructs for CRISPR-based gene targeting
- Identifying targets of PROTACs using mass spectrometry
- Basic principles and application of light microscopy and cryo-EM for structure/function analysis of proteins.
Competences
- Identify and evaluate advantages and limitations of the use of above listed methods.
- Justify the use of PROTACs and mass spectrometry in understanding the mechanisms of small molecule-based drugs or drug candidates
- Understand the central aspects of translational tools and be able to discuss and communicate these to other scientists, clinicians, and the public.
The course will be organized with a mix of scientific seminars
by invited speakers including lectures about stem cells, PROTACs,
CRISPR-based genome engineering, high-resolution microscopy,
cryo-EM, as well as the applications of modern proteomics
technologies for translational research.
In addition, the course will include group work, demonstrations,
and practical exercises supervised by leading specialists in the
field.
The course will end with an evaluation where participants must
reflect on course learning outcomes and give feedback for course
development.
Course literature will be published on Absalon.
Participants must meet the admission criteria in BRIDGE - Translational Excellence Programme.
The BRIDGE – Translational Excellence Programme offers a few selected graduated PhDs a two-year postdoctoral fellowship in translational medicine. The courses are only available to the fellows enrolled in the programme. Fellows are automatically enrolled in the courses upon admission to the programme.
For further information about the programme, please go to the BRIDGE website: www.bridge.ku.dk
- ECTS
- 0 ECTS
- Type of assessment
-
Continuous assessmentCourse participation
- Type of assessment details
- Attendance and active participation
- Exam registration requirements
-
Participants are automatically registered for the examination upon admission to the BRIDGE - Translational Excellence Programme.
- Aid
- All aids allowed
- Marking scale
- passed/not passed
- Censorship form
- No external censorship
Criteria for exam assessment
Active contribution and course participation according to the BRIDGE Guidelines and Practicalities.
Part time Master and Diploma courses
- Category
- Hours
- Lectures
- 10
- Preparation
- 5
- Theory exercises
- 5
- Exercises
- 20
- English
- 40
Kursusinformation
- Language
- English
- Course number
- SBRI19011U
- ECTS
- 0 ECTS
- Programme level
- Part Time Master
Ph.D.
- Placement
- Autumn
- Schedulegroup
-
See course dates and course programme in Absalon.
*This course is offered in autumn 2024 for the BRIDGE 2023-2025 programme. - Capacity
- 15 participants
- Studyboard
- Study Board for the Professionel Master´s Degree Programmes at The Faculty og Health and Medical Science
Contracting department
- Center for Protein Research
Contracting faculty
- Faculty of Health and Medical Sciences
Course Coordinators
- Chuna Ram Choudhary (15-666b78716431666b7278676b64757c43667375316e7831676e)
- Takeo Narita (12-7a67716b75347467786f7a674669767834717b346a71)
- Amelia Green (12-68746c737068356e796c6c75477a7c756b35727c356b72)
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