Translational Tools II – New Methods
Course content
The main themes covered in the course include:
- Stem cells, induced pluripotent stem cells, and their applications in disease modeling
- Precision nuclease-based genome editing
- Proteolysis targeting chimera (PROTACs)
- Mass spectrometry for understanding drug mechanisms
- High resolution microscopy
- Cryo-EM
BRIDGE - Translational Excellence Programme
On completion of the course, the participants should be able to:
Knowledge
- Explain the rationale for the utilization of several advanced
research methodologies to characterize protein mechanisms of action
in vivo with a focus on three general areas:
- Gene editing
- PROTACs
- Cryo-EM
- Discuss the applications of mass spectrometry-based proteomic approaches for drug target discovery
Skills
- Culture and use mouse embryonic stem cells
- Design of constructs for CRISPR-based gene targeting
- Identifying targets of PROTACs using mass spectrometry
- Basic principles and application of light microscopy and cryo-EM for structure/function analysis of proteins
Competences
- Identify and evaluate advantages and limitations of the use of above listed methods.
- Justify the use of PROTACs and mass spectrometry in understanding the mechanisms of small molecule-based drugs or drug candidates
- Understand the central aspects of translational tools and be able to discuss and communicate these to other scientists, clinicians, and the public
The course will be organized with a mixture of scientific seminars by invited speakers including lectures about stem cells, PROTACs, CRISPR-based genome engineering, high-resolution microscopy, cryo-EM, as well as the applications of modern proteomics technologies for translational research. In addition, the course will include group work, demonstrations, and practical exercises supervised by leading specialists in the field.
Course literature is published on Absalon.
Participants must meet the admission criteria in BRIDGE - Translational Excellence Programme.
The BRIDGE – Translational Excellence Programme offers a few select graduated PhDs a two-year postdoctoral fellowship in translational medicine. The courses are only available to the fellows enrolled in the programme. Fellows are automatically enrolled in the courses upon appointment in the programme.
For further information: https://bridge.ku.dk/about/
- ECTS
- 0 ECTS
- Type of assessment
-
Continuous assessmentCourse participation
- Type of assessment details
- Attendance and active participation.
- Aid
- All aids allowed
- Marking scale
- passed/not passed
- Censorship form
- No external censorship
Criteria for exam assessment
Active contribution and course participation according to the BRIDGE Guidelines.
Part time Master and Diploma courses
- Category
- Hours
- Lectures
- 10
- Preparation
- 5
- Theory exercises
- 5
- Exercises
- 20
- English
- 40
Kursusinformation
- Language
- English
- Course number
- SBRI19011U
- ECTS
- 0 ECTS
- Programme level
- Part Time Master
Ph.D.
- Duration
- Placement
- Autumn
- Schedulegroup
-
See course dates and course programme in Absalon.
*This course is offered in autumn 2023 for BRIDGE 2022-2024. - Capacity
- 15 participants
- Studyboard
- Study Board for the Professionel Master´s Degree Programmes at The Faculty og Health and Medical Science
Contracting department
- Center for Protein Research
Contracting faculty
- Faculty of Health and Medical Sciences
Course Coordinators
- Chuna Ram Choudhary (15-696e7b746734696e757b6a6e67787f4669767834717b346a71)
- Takeo Narita (12-77646e6872317164756c776443667375316e7831676e)
- Marie Sofie Yoo Tollenaere-Larsen (12-7064756c68316f647576687143667375316e7831676e)
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Courseinformation of students