Gene Technology (Gentek)

Course content

The theoretical part includes:

  • A thorough review of gene technology methods
  • Cellular control of macromolecular biosynthesis with emphasis on the experimental basis
  • Generation of genome- and cDNA libraries
  • Screening of libraries including expression cloning in Xenopus oocytes and micro organisms
  • Genome-, trancriptome- and proteome analysis
  • Production of transgenic animals
  • Heterologous expression in bacteria, yeast/fungi, insect cells, mammalian cells and whole animals


The practical part includes:

  • The most important methods to generate recombinant plasmids using restriction enzymes, Gateway® technology or homologous recombination
  • Site-directed mutagenesis
  • Real-time PCR for mRNA quantification and SNP analysis
  • Application of reporter genes to study gene regulation and subcellular protein localisation
  • Yeast 2-hybrid analysis
  • Affinity purification of fusion proteins
  • Pull-down experiments
  • Fluorescence microscopy
  • Internet based gene technology tools
  • Danish and EU law on gene technology
Education

BSc Programme in Biochemistry
BSc Programme in Biology

Learning outcome

Knowledge:

The course provides the student with a detailed and comprehensive knowledge on theoretical and practical modern gene technology. The student gets aquainted with practical laboratory routines fulfilling the requirements stated by the Danish and EU law on gene technology.

Skills:

The student gains core competences within theoretical and practical modern gene technology. The degree of confidence with modern gene technology/molecular biology obtained during the course allows him/her to design gene technology/molecular biology experiments based upon known gene technological methods and internet based tools. The student obtains experience with critical interpretation and treatment of original molecular biology data, and gets acquainted with work routines in a gene technology laboratory in relation to Danish and EU law on gene technology and genetically modified organisms (GMOs).
Practical: The student gets aquainted with practical laboratory routines fulfilling the requirements stated by the Danish and EU law on gene technology.
Personal: The student learns to collaborate in groups and prepare a written presentation and discussion of their obtained experimental data.

Competencies:

The competencies obtained by the student include

  • to interpret data from the scientific literature and put them in a molecular biology/gene technology context
  • to design their own experiments
  • to work in a classified laboratory in accordance with legal regulations
  • to work in small teams
  • to give a detailed description of and evaluate the gene technology tools presented during the course
  • to apply the presented tools to design experiments with the potential to answer defined molecular biology questions
  • to describe and explain individual steps in a presented gene technology manual

Lectures, problem solving and laboratory experiments.

See Absalon.

Knowledge corresponding to the content of the courses: Basic Biochemistry, Basic Molecular Biology and Basic Cell Biology.

The course is offered to students of Biology, Biochemistry, Molecular Biomedicine, Nanotechnology, Sports Science and related educations.

Written
Collective
ECTS
15 ECTS
Type of assessment
Written examination, 4 hours under invigilation
Type of assessment details
---
Aid
All aids allowed
Marking scale
7-point grading scale
Censorship form
No external censorship
One internal examiner
Criteria for exam assessment

In order to obtain the grade 12 the student should convincingly and accurately demonstrate the knowledge, skills and competences described under Learning Outcome.

 

Single subject courses (day)

  • Category
  • Hours
  • Lectures
  • 28
  • Class Instruction
  • 28
  • Preparation
  • 254
  • Practical exercises
  • 90
  • E-Learning
  • 8
  • Exam
  • 4
  • English
  • 412

Kursusinformation

Language
English
Course number
NBIB10009U
ECTS
15 ECTS
Programme level
Bachelor
Duration

1 block

Placement
Block 1
Schedulegroup
All schedules
Capacity
No limit
The number of seats may be reduced in the late registration period
Studyboard
Study Board for the Biological Area
Contracting department
  • Department of Biology
Contracting faculty
  • Faculty of Science
Course Coordinator
  • Per Amstrup Pedersen   (10-7869786d6c6d7a7b6d76486a717736737d366c73)
Teacher

Rasmus Hartmann-Petersen og Henriette Pilegaard

Saved on the 28-02-2022

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