Translational Tools II – New Methods

Course content

The main themes covered in the course include:

  • Stem cells, induced pluripotent stem cells, and their applications in disease modeling
  • Precision nuclease-based genome editing
  • Proteolysis targeting chimera (PROTACs)
  • Mass spectrometry for understanding drug mechanisms
  • High resolution microscopy
  • Cryo-EM
Education

BRIDGE - Translational Excellence Programme

A two-year postdoctoral fellowship in translational medicine

Learning outcome

On completion of the course, the participants should be able to:

 

Knowledge

  • Explain the rationale for the utilization of several advanced research methodologies to characterize protein mechanisms of action in vivo with a focus on three general areas: 
    • Gene editing
    • PROTACs
    • Cryo-EM
  • Discuss the applications of mass spectrometry-based proteomic approaches for drug target discovery

 

Skills

  • Culture and use mouse embryonic stem cells
  • Design of constructs for CRISPR-based gene targeting
  • Identifying targets of PROTACs using mass spectrometry
  • Basic principles and application of light microscopy and cryo-EM for structure/function analysis of proteins

 

Competences

  • Identify and evaluate advantages and limitations of the use of above listed methods.
  • Justify the use of PROTACs and mass spectrometry in understanding the mechanisms of small molecule-based drugs or drug candidates
  • Understand the central aspects of translational tools and be able to discuss and communicate these to other scientists, clinicians, and the public

 

The course will be organized with a mixture of scientific seminars by invited speakers including lectures about stem cells, PROTACs, CRISPR-based genome engineering, high-resolution microscopy, cryo-EM, as well as the applications of modern proteomics technologies for translational research. In addition, the course will include group work, demonstrations, and practical exercises supervised by leading specialists in the field.

Course literature is published on Absalon.

 

Course literature includes:

The next generation of CRISPR-Cas technologies and applications. Pickar-Oliver A, Gersbach CA. Nat Rev Mol Cell Biol. 2019 Aug;20(8):490-507. PMID: 31147612

 

PROTACs: An Emerging Therapeutic Modality in Precision Medicine. Dhanusha A. Nalawansha and Craig M. Crews. Cell Chemical Biology. 2020, 27(8):998-1014.

 

Chemoproteomics and Chemical Probes for Target Discovery. Drewes G1, Knapp S2. Trends Biotechnol. 2018 Dec;36(12):1275-1286. PMID: 30017093

 

Induced pluripotent stem cells in disease modelling and drug discovery. Rowe RG, Daley GQ. Nat Rev Genet. 2019 Jul;20(7):377-388. PMID: 30737492

 

Microscopy-Based High-Content Screening. Boutros M, Heigwer F, Laufer C. 2015. Cell 163(6):1314-25. doi: 10.1016/j.cell.2015.11.007.

Participants must meet the admission criteria in BRIDGE - Translational Excellence Programme

Oral
Continuous feedback during the course of the semester
ECTS
0 ECTS
Type of assessment
Continuous assessment
Course participation
Attendance and active participation
Aid
All aids allowed
Marking scale
passed/not passed
Censorship form
No external censorship
Criteria for exam assessment

Active contribution and course participation according to the BRIDGE Guidelines.

Part time Master and Diploma courses

  • Category
  • Hours
  • Lectures
  • 10
  • Preparation
  • 5
  • Theory exercises
  • 5
  • Exercises
  • 20
  • English
  • 40