Translational Discovery Omics II

Course content

The aim of the course is to provide the participants with: i) an overview of the major high-end quantitative proteomics technologies with focus on mass spectrometry; ii) an overview of the wealth of biological applications that quantitative proteomics screens can be applied in, including proteome quantitation post translational modification (PTM) analysis and protein-protein interactions; and iii) introduction on how to process proteomics data at different levels.

 

The main themes covered in the course include:

  • Mass spectrometry technologies and principles for protein sequencing.
  • Introduction to proteomics
  • Applications of quantitative proteomics in biology. The use of proteomics in industry and academia.

 

Education

BRIDGE - Translational Excellence Programme

A two-year postdoctoral fellowship in translational medicine

 

Learning outcome

Upon completing the course, participants should be able to:

 

Knowledge

  • Demonstrate a basic understanding of different mass spectrometry-based proteomics workflows and methods.
  • Gain a general understanding of the main statistical concepts required for proteomics analysis.
  • Comprehend different models of proteomics analysis in different research settings.
  • Grasp the role of protein signaling pathways and protein-protein interactions in health and disease.

 

Skills and Competences

  • Be able to perform mass spectrometry of biological based proteomics analysis of biological samples.
  • Process raw mass spectrometry data and obtain peptide and protein identification from proteomics analysis using different software tools.
  • Use data analysis and visualization programs such as MaxQuant, Spectronaut, Perseus, R, and Cytoscape and integrate proteomics data with curated datasets such as signaling pathways and protein-protein interactions.
  • Understand the central aspects of translational omics and be able to discuss and communicate these to other scientists, clinicians, and the public
  • Critically evaluate the results and interpretation of downstream data analysis to answer biological questions and hypotheses.

 

The course is organized with a mixture of student-led lectures and scientific seminars by invited speakers including technical lectures about modern proteomics technologies used to study cellular signalling pathways, protein-protein interactions and global proteome changes. In addition, the course will include group work, case studies/journal clubs, demonstrations, and practical exercises provided and supervised by the world leading specialists in the field.

Course literature is published on Absalon.

Participants must meet the admission criteria in BRIDGE - Translational Excellence Programme

Oral
Continuous feedback during the course of the semester
ECTS
0 ECTS
Type of assessment
Continuous assessment
Course participation
Attendance and active participation
Aid
All aids allowed
Marking scale
passed/not passed
Censorship form
No external censorship
Criteria for exam assessment

Active contribution and course participation according to the BRIDGE Guidelines.

Part time Master and Diploma courses

  • Category
  • Hours
  • Lectures
  • 8
  • Class Instruction
  • 16
  • Preparation
  • 5
  • Theory exercises
  • 4
  • English
  • 33