Experimental Molecular Biology

Course content

The course offers experimental platforms to acquire hands-on experience with techniques relevant to biotechnology. The choice of topics ensures that the students learn about standard methods in molecular biology and biochemistry. Additionally, the course comprises an introduction to how to critically read and interpret scientific literature.

The students will gain experience with a wide-range of both basic and advanced experimental methods in molecular biology, e.g. extraction of DNA and RNA, PCR, RT-PCR, real-time PCR, cloning, primer and vector design, transformation of prokaryotic and eukaryotic organisms, heterologous gene expression, protein purification, immunoblotting, histochemical analyses, enzyme kinetics, bioimaging and basic bioinformatics and data analysis. The organisms employed in the exercises include bacteria, yeast, and plants to emphasize that the methods and basic scientific principles taught have general relevance.

Education

BSc Programme in Biotechnology

Learning outcome

The course aims at providing practical experience and theoretical knowledge about basic principles and methods in experimental molecular biology.

After completing the course the students should be able to:

Knowledge:

  • Describe basic principles and analytical methods used in molecular biology research.
  • Explain how these principles and methods can be exploited in practical experiments adressing research questions within biotechnology.

 

Skills:

  • Carry out experiments using a number of general methods in molecular biology, biochemistry and cell biology.
  • Analyse, visualize, interpret and conclude from results acquired in the laboratory.
  • Summarise and present scientific concepts and own experimental results to fellow scientists through written and oral communication.
  • Interpret and discuss results presented in scientific articles within the field of molecular biology and biotechnology.
  • Use e.g digital tools in creative processes in the context of project management and presentation.

 

Competences:

  • Work in a groups to plan and carry out defined experimental work.
  • Reflect upon the empiric basis of research within the field of biotechnology through critical reading of original research articles.

 

The course comprises lab exercises with written lab reports, lectures and journal clubs. The students carry out different experiments as specified in the description of the exercises. The results of the exercises are discussed in follow-up sessions. The exercises are complemented by lectures and journal clubs, which provide the theoretical background for the experiments.

Literature

Laboratory manuals including theoretical background for each practical exercise provided on Absalon together with the research articles discussed in the journal clubs. The literature changes from year to year dependent on the chosen topics.

Text book:

“Principles and Techniques of Biochemistry and Molecular Biology Paperback”, Keith Wilson (Editor) and John Walker (Editor).

Participation in courses which provide basic knowledge in biochemistry and molecular genetics is required.

Written
Oral
Continuous feedback during the course of the semester
Peer feedback (Students give each other feedback)
ECTS
15 ECTS
Type of assessment
Written examination, 4 hours under invigilation
Exam topics include theories and methods taught in theoretical and practical exercises, lectures, and journal clubs.
Aid
All aids allowed
Marking scale
7-point grading scale
Censorship form
External censorship
Criteria for exam assessment

In order to obtain the grade 12 the student should convincingly and accurately demonstrate the knowledge, skills and competences described under Learning Outcomes.

Single subject courses (day)

  • Category
  • Hours
  • Lectures
  • 32
  • Class Instruction
  • 22
  • Preparation
  • 210
  • Theory exercises
  • 14
  • Practical exercises
  • 130
  • Exam
  • 4
  • English
  • 412