Translational Tools II – New Methods

Course content

The main themes covered in the course include:

  • High resolution microscopy
  • Induced pluripotent stem cells
  • Protein editing
  • Gene editing/CRISPR-Cas9
  • mab
  • Cryo EM
  • Tumography
  • Stem cell biology
Education

BRIDGE - Translational Excellence Programme

 

Learning outcome

On completion of the course, the participants should be able to:

 

Knowledge

  • Explain the rationale for the utilization of several advanced research methodologies to characterize protein mechanisms of action in vivo with a focus on two general areas: 

                1) light microscopy-based analysis of protein signalling and                                protein- protein interactions,

                2) gene editing

  • Discuss the principles of antibody-based methods to study chromatin regulation and function in living cells and tissues.

 

Skills

  • Use sample preparation methods for the analysis of protein posttranslational modifications.
  • Analyse the physical principles that govern the use of light microscopy methods for the characterization of proteins in living cells.
  • Apply different microscopy methods commonly used for analysis of cellular protein signalling events, including the use of advanced imaging analysis and live cell imaging.

 

Competences

  • Identify and evaluate advantages and limitations of the use of above listed methods.
  • Justify the use of cryo-electron microscopy (EM) in protein structure determination
  • Understand the central aspects of translational tools and be able to discuss and communicate these to other scientists, clinicians, and the public

The course is organized with a mixture of student-led lectures and scientific seminars by invited speakers including technical lectures about modern proteomics technologies used to study cellular signalling pathways, protein-protein interactions and global proteome changes. In addition, the course will include group work, case studies/journal clubs, demonstrations, and practical exercises provided and supervised by the world leading specialists in the field.

Boutros M, Heigwer F, Laufer C. Microscopy-Based High-Content Screening. 2015. Cell 163(6):1314-25. doi: 10.1016/j.cell.2015.11.007.

 

Cheng JK, Alper HS. The genome editing toolbox: a spectrum of approaches for targeted modification. 2014. Curr Opin Biotechnol; 30:87-94. doi: 10.1016/j.copbio.2014.06.005. Epub 2014 Jul 8.

 

Neumann B1, Walter T, Hériché JK, Bulkescher J, Erfle H, Conrad C, Rogers P, Poser I, Held M, Liebel U, Cetin C, Sieckmann F, Pau G, Kabbe R, Wünsche A, Satagopam V, Schmitz MH, Chapuis C, Gerlich DW, Schneider R, Eils R, Huber W, Peters JM, Hyman AA, Durbin R, Pepperkok R, Ellenberg J. Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. 2010. Nature; 464(7289):721-7. doi: 10.1038/nature08869.

Participants must meet the admission criteria in BRIDGE - Translational Excellence Programme

Oral
Continuous feedback during the course of the semester
ECTS
0 ECTS
Type of assessment
Continuous assessment
Course participation
Attendance and active participation
Aid
All aids allowed
Marking scale
passed/not passed
Censorship form
No external censorship
Criteria for exam assessment

Knowledge

  • Understand the principles of high resolution microscopy
  • Understand the principles of gene editing
  • Understand the basis for the analysis of protein-protein interactions in living cells using advanced microscopy techniques such as FRAP and FRET

 

Skills

  • Interpret the experimental data obtained by the above-mentioned methods.
  • Present experimental data in an understandable and scientifically sound manner.
  • Discuss and critically review articles and research proposal for appropriateness in the utilization of methodological approaches for characterization of protein function in vivo.
  • Design a tailored research plan to test specific hypotheses related to the characterization of protein function in mammalian cells using relevant and informative methods

 

Competences

  • Project planning,
  • Data acquisition and interpretation,
  • Scientific communication both written and oral

Part time Master and Diploma courses

  • Category
  • Hours
  • Lectures
  • 10
  • Theory exercises
  • 5
  • Exercises
  • 20
  • Course Preparation
  • 5
  • English
  • 40