Experimental Molecular Biology

Course content

The course offers experimental platforms to acquire new skills with regard to methods in molecular biology and biotechnology. The choice of topics ensures that the students learn standard techniques relevant to molecular biology, biochemistry and physiology. Additionally, the course comprises an introduction to how to critically read and evaluate scientific literature.

The students will gain experiences in a wide-range of both basic and advanced experimental methods in molecular biology, e.g. extraction of DNA and RNA, PCR, RT-PCR, real-time PCR, cloning, primer and vector design, transformation of prokaryotic and eukaryotic organisms, cultivations of cell cultures, heterologous gene expression, protein purification, immunoblotting, histochemical analyses, enzyme kinetics, and bioimaging and basic bioinformatics. A wide-range of experimental organisms are employed including bacteria, yeast, fungi, plants and mammalian cells to emphasize that the methods and basic scientific principles taught have general relevance.


BSc Programme in Biology-Biotechology

Learning outcome

The course aims at providing practical experience and theoretical knowledge of basic principles and methods in experimental molecular biology.

After completing the course the students should be able to:


  • Describe basic principles and analytical methods used in molecular biology research.
  • Explain how these principles and methods can be exploited in practical experiments aimed at reaching distinct research goals.



  • Carry out experiments using a number of general methods in molecular biology, biochemistry and cell biology.
  • Analyse, interpret and conclude from results acquired in the laboratory.
  • Summarise and present scientific concepts and own experimental results to fellow scientists through written and oral communications.
  • Interpret and discuss results presented in scientific articles within the field of molecular biology and biotechnology.
  • Use e.g IT tools in creative processes in the context of project management and presentation.



  • Work independently as well as together with other students to plan and carry out defined experimental work.
  • Reflect upon the empiric basis of research within the field of biotechnology through critical reading of original research articles.

The course comprises lab exercises with written lab reports, lectures and journal clubs. The students carry out different experiments as specified in the description of the exercises. The results of the exercises are discussed in follow-up sessions. The exercises are complemented by lectures and journal clubs in the mornings, which provide the theoretical background for the experiments.


Laboratory manuals including theoretical background for each practical exercise provided on Absalon together with the research articles discussed in the journal clubs and a compendium for ethics and philosophy of science. The literature changes from year to year dependent on the chosen topics.

Text book:

“Principles and Techniques of Biochemistry and Molecular Biology Paperback”, Keith Wilson (Editor) and John Walker (Editor).

Participation in courses which provide basic knowledge in biochemistry and molecular genetics is required.

Continuous feedback during the course of the semester
Peer feedback (Students give each other feedback)
Type of assessment
Written examination, 4 hours under invigilation
Exam topics include theories and methods taught in theoretical and practical exercises, lectures, and journal clubs.
The course has been selected for ITX exam on Peter Bangs Vej.
All aids allowed

The University will make computers and power available to students taking written exams with invigilation in the University’s building on Peter Bangs Vej 36 (ITX). Students are therefore not permitted to bring their own computers, tablets or mobile phones. If textbooks and/or notes are permitted, according to the course description, these must be in paper format or on a USB flash drive.

Marking scale
7-point grading scale
Censorship form
External censorship
Criteria for exam assessment

In order to obtain the grade 12 the student should convincingly and accurately demonstrate the knowledge, skills and competences described under Learning Outcomes.

Single subject courses (day)

  • Category
  • Hours
  • Exam
  • 4
  • Preparation
  • 210
  • Lectures
  • 32
  • Colloquia
  • 22
  • Practical exercises
  • 130
  • Theory exercises
  • 14
  • English
  • 412