Gene Technology - Nano (GentekN)

Course content

The course includes:

  • A thourough review of gene technology methods
  • Cellular control of macromolecular biosynthesis with emphasis on the experimental basis
  • Generation of genome- and cDNA libraries
  • Screening of libraries including expression cloning in Xenopus oocytes and Saccharomyces cerevisiae
  • Genome-, trancriptome- and proteome analysis
  • Production of transgenic animals
  • Heterologous expression in bacteria, yeast/fungi, insect cells, mammalian cells and whole animals
Education

BSc Programme in Nanoscience
BSc Programme in Molecular Biomedicine

Learning outcome

Knowledge:
The student will obtain detailed knowledge on

  • gene technology methods
  • cellular control of macromolecular biosynthesis with emphasis on the experimental basis
  • generation of genome- and cDNA libraries
  • screening of libraries including expression cloning in Xenopus oocytes and Saccharomyces cerevisiae
  • genome-, trancriptome- and proteome analysis
  • production of transgenic animals
  • heterologous expression in bacteria, yeast/fungi, insect cells, mammalian cells and whole animals


Skills:
The student gains core competences within modern gene technology. The degree of confidence with modern gene technology/molecular biology obtained during the course allows him/her to design gene technology/molecular biology experiments based upon known gene technological methods. The student obtains experience with critical interpretation and treatment of original molecular biology data.

Competences:
The two most important competences obtained by the student are to comprehend scientific data and put them in a genetechnology/molecular biology context and to use the genetechnology toolbox to solve real genetechnological/molecular biology problems.

Lectures, problem solving.

See Absalon.

The course is offered to students in Nanotechnology and related educations. Knowledge corresponding to the content of the course: Nanobio1.

ECTS
7,5 ECTS
Type of assessment
Written examination, 4 hours under invigilation
The course has been selected for ITX exam at Peter Bangs Vej
Aid
All aids allowed

NB: If the exam is held at the ITX, the ITX will provide you a computer. Private computer, tablet or mobile phone CANNOT be brought along to the exam. Books and notes should be brought on paper or saved on a USB key.

Marking scale
7-point grading scale
Censorship form
No external censorship
One internal examiner
Criteria for exam assessment

To obtain the mark 12 the student should

  • Be able to give a detailed description of and evaluate the gene technology tools presented during the course
  • Be able to apply the presented tools to design experiments with the potential to answer defined molecular biology questions
  • Be able to describe and explain individual steps in a presented gene technology manual
  • Be able to interpret data from the scientific literature and relate those to the content of the course

Single subject courses (day)

  • Category
  • Hours
  • Exam
  • 4
  • Lectures
  • 28
  • Colloquia
  • 28
  • Preparation
  • 146
  • English
  • 206