Kursussøgning, efter- og videreuddannelse – Københavns Universitet

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Kursussøgning, efter- og videreuddannelse

In-vitro Techniques in Biochemistry and Pharmacology

Practical information
Study year 2016/2017
Time
Block 1
Programme level Full Degree Master
Full Degree Master choice
Part Time Master
ECTS 7,5 ECTS
Course responsibles
  • Darryl S Pickering (6-726b656d67744275777066306d7730666d)
  • Harald S. Hansen (3-7883785083857e743e7b853e747b)
  • Department of Drug Design and Pharmacology
Course number: SFKKB9091U

Course content

The following practical elements are included: primary cell cultures, neurotransmitter transporters, receptor activation (GPRC and ligand-gated ion-channel receptors), receptor binding, electrophysiology, second messenger measurements, FRET, enzyme fractionation, enzyme inhibition, smooth muscle contraction, CytP450 and drug metabolism, separation of lipophilic drug metabolites, use of radioisotopes, drug effects on isolated mitochondria.

Learning outcome

By the end of the course, the students will have acquired the competences to:

Knowledge

  • demonstrate a firm knowledge of in vitro pharmacology 
  • understand common in vitro experimental methodologies as applied in pharmacology.

 

Skills

  • The students will acquire practical and theoretical abilities and skills in pharmacological in vitro research techniques that are applicable in both academic and biotech/pharmaceutical drug discovery research environments. The specific aim is to acquire the abilities and practical skills required to be able to work in a pre-clinical drug discovery research environment and/or communicate proficiently with other professionals.
  • prepare cultures of primary mouse neurons
  • execute neurotransmitter uptake experiments employing cultured neurons
  • execute experiments to elucidate drug-induced contraction of the superior mesenteric artery isolated from rats
  • measure cAMP (second-messenger) in cultured cells by FRET
  • employ isolated mouse vas deferens to measure the effect of morphine-like pain-relieving drugs
  • employ two-electrode voltage clamp (TEVC) electrophysiology technique as a functional assay of drugs affecting the operation of ion channels
  • employ radioligand binding assays in drug investigations and interpret the resulting pharmacologial data
  • purify an enzyme employing affinity chromatography
  • perform enzyme inhibition assays
  • analyze cytochrome P450-mediated metabolism of a radiolabeled drug in a liver homogenate employing thin layer chromatography (TLC) and subsequent scintillation counting
  • isolate crude mitochondria from a rat brain or liver homogenate
  • employ isolated mitochondria to perform a simple in vitro screen of toxicity related to mitochondrial function of unknown drugs.

 

Competences

  • work with and/or communicate proficiently with other professionals within the field of in vitro pharmacology
  • design simple in vitro assays of drug activity including choosing the proper model system and assay conditions
  • calculate and interpret pharmacological data (dose-response relationships)
  • apply knowledge of in vitro pharmacology to evaluate pharmacological data in relation to the drug discovery process
  • calculate and interpret pharmacological data (dose-response relationships)
  • apply knowledge of in vitro pharmacology to evaluate pharmacological data in relation to the drug discovery process

Recommended prerequisites

Students should have completed bachelor’s level lecture course(s) in biochemistry and/or pharmacology as well as a lab course in chemistry and/or biochemistry.

Mandatory prerequisites

None.

Remarks

The schedule for this course is not compatible with that of Clinical Pharmacy Practice (SFKKB9011U).

Sign up

Open for Danish and international guest students. For application details look here: http://sund.ku.dk/uddannelse/andre-uddannelsestilbud/meritstuderende/

 

 

 

Education

M.Sc. in Pharmaceutical Sciences - elective

M.Sc. in Medicinal Chemistry - elective

Cand. Scient. Pharm. - elective

Cand. Pharm. - elective

MSc Programme in Environmental Science - restricted elective

 

Studyboard

Study Board of Pharmaceutical Sciences

Teacher

Announced at the course start.

Duration

1 block

Schedulegroup

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Teaching and learning methods

Lab exercises: 48 hours
Tutorials: 13 hours

Capacity

56 students

Language

English

Literature

  • Lab manual (Pickering and Hansen)
  • Receptor theory notes (Sheykhzade and Pickering)
  • Radioisotope theory notes (Hansen and Pickering)

Workload

Category Hours
Practical exercises 48
Colloquia 26
Lectures 2
Preparation 80
Exam Preparation 50
English 206

Exam (SFKKB9091E - written examination)

Type of assessment

Written examination, 2 hours under invigilation
Examination design
The course examination will consist of 20 short-answer questions covering the practical and theoretical aspects of all the laboratory exercises. All written answers will be given an equal weighting and then the course leaders will assess the overall examination performance level.

Aid

No aids are allowed

Refering to the IT exams homepage link where the standard programs and IT tools available for the students’ disposal during IT exams under The Faculty of Health and Medical Sciences are listed.

Marking scale

7-point grading scale

Criteria for exam assessment

To achieve the grade 12 the student must be able to:

Knowledge

  • demonstrate a firm knowledge of in vitro pharmacology 
  • understand common in vitro experimental methodologies as applied in pharmacology.

 

Skills

  • prepare cultures of primary mouse neurons
  • execute neurotransmitter uptake experiments employing cultured neurons
  • execute experiments to elucidate drug-induced contraction of the superior mesenteric artery isolated from rats
  • measure cAMP (second-messenger) in cultured cells by FRET
  • employ isolated mouse vas deferens to measure the effect of morphine-like pain-relieving drugs
  • employ two-electrode voltage clamp (TEVC) electrophysiology technique as a functional assay of drugs affecting the operation of ion channels
  • employ radioligand binding assays in drug investigations and interpret the resulting pharmacologial data
  • purify an enzyme employing affinity chromatography
  • perform enzyme inhibition assays
  • analyze cytochrome P450-mediated metabolism of a radiolabeled drug in a liver homogenate employing thin layer chromatography (TLC) and subsequent scintillation counting
  • isolate crude mitochondria from a rat brain or liver homogenate
  • employ isolated mitochondria to perform a simple in vitro screen of toxicity related to mitochondrial function of unknown drugs.

 

Competences

  • work with and/or communicate proficiently with other professionals within the field of in vitro pharmacology
  • design simple in vitro assays of drug activity including choosing the proper model system and assay conditions
  • calculate and interpret pharmacological data (dose-response relationships)
  • apply knowledge of in vitro pharmacology to evaluate pharmacological data in relation to the drug discovery process

Censorship form

No external censorship
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