Kursussøgning, efter- og videreuddannelse – Københavns Universitet

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Kursussøgning, efter- og videreuddannelse

Advanced Analytical Chemistry - Chromatography and Mass Spectrometry

Practical information
Study year 2016/2017
Block 2
Programme level Full Degree Master
Course responsible
  • Nikoline Juul Nielsen (3-7e7a7e50807c757e3e7b853e747b)
  • Department of Plant and Environmental Sciences
Course number: NPLK13004U

Course content

Today, chromatography and mass spectometry is by far the most common used analytical techniques. A thorough understanding of the chromatographic theory, and operating mechanism of ionisation techniques and mass analysers is essential for method selection, development and optimisation.

The course covers chromatographic separation of organic compounds with special emphasize on the molecular mechanism and theory of analyte-column interactions for gas and liquid chromatography, and the theory of ionization, fragmentation, mass separation, detection and data-analysis for all kind of mass spectrometers and ionization techniques in common. All common analytical instruments and techniques from GC-FID/MS to UPLC-UV/FLU/MSMS are addressed. The theory is supplemented with computer simulations and expert guest-lectures of selected topics.

Learning outcome

After completing the course the student should have acquired:

Knowledge - the student has:
-A solid theoretical understanding of chromatography and mass spectrometry at an advanced level.
-Experience in adressing complex analytical problems, and acquaintance with tools to solve them.

Skills - The student will be able to:
-Select suitable analytical platform for the separation, detection and quantification of analytes from complex matrices including selection and assessment of column, mobile phase and chromatographic parameter; ionisation mode, source and mass analyser parameterisation.
-Do exact mass measurements and fragmentation experiments.
-Troubleshoot advanced chromatographic and mass spectrometric equipment.
-Evaluate the validity of an analytical method and its application domain.
-Critically assess reported analytical chemistry research.

Competences - The student can be considered a:
-Matured analytical chemist with the ability to sketch, develop, implement, maintain and evaluate any chromatographic mass spectrometric method for analysis of complex mixtures.

Recommended prerequisites

The course LKEF10076 Analytical Chemistry or equivalent is a requirement, since all basic theory of separation and mass spectrometry is assumed to be known.

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MSc Programme in Environmental Science
MSc Programme in Chemistry


Study Board of Physics, Chemistry and Nanoscience

Course type

Single subject courses (day)


Ass. prof. Nikoline Juul Nielsen and others


1 block


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Teaching and learning methods

Lectures, student presentations of curriculum, expert lectures, excursions, hands-on experiments, theoretical exercises, computer simulations.


A maximum of 30 participants




Selected articles will be used. The textbooks will be announced on Absalon, for inspiration consult:

"Introduction to Modern Liquid Chromatography", by Lloyd R. Snyder; Joseph J. Kirkland and John W. Dolan, 3rd Ed., Wiley 2009.

"Mass Spectrometry. Principles and Applications", by Edmond de Hoffmann and Vincent Stroobant, 3rd Ed., Wiley 2007.


Category Hours
Preparation 123
Lectures 16
Practical exercises 11
Theory exercises 10
Colloquia 12
Excursions 8
Exam 10
Project work 16
English 206


Type of assessment

Oral examination, 30 min


Only certain aids allowed

All written aids and any calculator which does not allow communication

Marking scale

7-point grading scale

Criteria for exam assessment

See learning outcome.

Censorship form

No external censorship
One internal examiner


There can not be dispensated for the requirement of laboratory exercises, and students who do not fulfil the requirement has to follow the course the following study year.

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