Kursussøgning, efter- og videreuddannelse – Københavns Universitet

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Kursussøgning, efter- og videreuddannelse

Genome Sequence Analysis

Practical information
Study year 2016/2017
Time
Block 2
Programme level Full Degree Master
ECTS 7,5 ECTS
Course responsible
  • Robin Andersson (5-7774676e7345676e7433707a336970)
  • Department of Biology
Course number: NBIK15013U

Course content

The course will introduce contemporary methods for analyzing whole genome sequnecing (WGS) data generated by next generation sequencing (NGS) platforms. The theoretical framework for DNA shearing, linker addition, immobilization and solid-phase amplification will be explained. Algorithms for mapping sequence reads onto a reference model genome (yeast) will be covered. Based on authentic biological samples, mutants will be identified by variant calling. Genetic experiments that relate the identified mutants to a biological phenotype will be conducted.

Learning outcome

Knowledge:

  • Next generation sequencing techniques
  • Statistics of sequencing coverage
  • Methods for mapping of sequencing runs onto a reference genome
  • Variant calling algorithms
  • Relationship between phenotypic and genotypic segregation


Skills:

  • Design experiment to map mutations by whole genome sequencing
  • Analyse, interpret and critically evaluate WGS data
  • Map WGS data onto a reference genome
  • Identify mutations in an experimental sample by variant calling
  • Design genetic experiments to relate identified variants with mutants phenotypes


Competences:

  • The students will be able to critically evaluate results of WGS experiments from different perspectives (molecular biology, bioinformatics, genetics)

Recommended prerequisites

BSc in Biology, Bioinformatics, Biochemistry, Molecular Biomedicine or equivalent.

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Education

MSc Programme in Biochemistry
MSc Programme in Biology
MSc Programme in Molecular Biomedicine
MSc Programme in Biology-Biotechnology

Studyboard

Study Board of Biology and Animal Science

Course type

Single subject courses (day)

Teacher

Robin Andersson, Olaf Nielsen, Anders Albrechtsen

Duration

1 block

Schedulegroup

C
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Teaching and learning methods

Theoretical and practical exercises, colloquia.

Capacity

24

Language

English

Literature

Notes, articles, biological material.
See Absalon.

Workload

Category Hours
Preparation 131
Theory exercises 21
Practical exercises 42
Colloquia 12
English 206

Exam

Type of assessment

Continuous assessment
Participation in the theoretical and practical exercises and colloquia.

Marking scale

passed/not passed

Criteria for exam assessment

See Learning Outcome.

Censorship form

No external censorship
One internal examiner

Re-exam

The reexam will be held as an oral exam (30 minutes) with 1 hour preparation time (all aids allowed).

 

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