Molecular Biotechnology

Course content

The theoretical part includes:

  • A thorough review of organisms used for recombinant protein production
  • Protein purification related to recombinant protein production.
  • Quality control of recombinant proteins
  • Protein quality in relation to application
  • Optimization of recombinant protein production
  • Large scale growth of host organism
  • Industrial recombinant protein production with experts from Industry
Education

MSc Programme in Biology
MSc Programme in Biochemistry

Learning outcome

Knowledge:

Molecular Biotechnology focuses on how to apply a thorough knowledge on fundamental molecular biology, genetics and protein chemistry to rationalize recombinant protein production on a lab scale as well as on an industrial scale. The student will gain in depth knowledge on the organisms used in industry for recombinant protein production. This will include bacteria, eukaryotic microorganisms and mammalian cell lines as well as intact organisms. The student will obtain knowledge to select the most optimal host cell for production of a specific protein.

Skills:

The student gains core competences within recombinant protein production as an important tool in the research laboratory and a billion dollar business for the biotech industry. A number of different cell factories are available for recombinant protein production. The degree of confidence with recombinant protein production obtained during the course allows the student to evaluate benefits and draw backs related to selection of host organism and protein purification. The student will get familiar with how to optimize recombinant protein production by applying methods from molecular biology, genetics and protein science. The student will be able to critically evaluate the potential of a specific host organism in relation to the intended application of the recombinant protein.

Competences:

The competences obtained by the student include

  • to interpret and evaluate data from the scientific literature related to recombinant protein production

  • to design recombinant protein production and purification experiments

  • to select an expression host compatible with desired quality of the recombinant protein product

  • to evaluate possibilities for setting up recombinant protein production on a laboratory and industrial scale

  • to make a literature study on a subject related to recombinant protein production and make an oral presentation of the outcome.

Lectures, colloquia

See Absalon

Knowledge corresponding to the content of the courses: Biochemistry 2, Biochemistry 3, Genetechnology, Genetechnology-nano, Protein Science or equivalent.
The course is offered to students of Biology, Biochemistry, Molecular Biomedicine, Nanotechnology, Sports Science and related educations.

ECTS
7,5 ECTS
Type of assessment
Written assignment, 1 week
Written take-home assignment, 1 week.
Marking scale
7-point grading scale
Censorship form
No external censorship
One internal examiner
Criteria for exam assessment

To pass the student should

  • Be able to give a detailed description of and evaluate the host organisms presented during the course for recombinant protein production.
  • Be able to thoroughly evaluate experimental data from the scientific literature
  • Be able to thoroughly present data from the scientific literature on recombinant protein production

Single subject courses (day)

  • Category
  • Hours
  • Lectures
  • 28
  • Preparation
  • 150
  • Colloquia
  • 28
  • English
  • 206